The overall objective of this work package is the implementation of enzymatic routes enabling a designer P. putida strain to metabolize the PET and PU monomers, arising from their depolymerisation, into central building blocks. All heterologous metabolic pathways will be integrated using the SEVA standard to enable efficient consolidation, as not only the conversion of individual chemicals is required, but also the dynamic integration of multi-substrate utilisation into the P4SB host.